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White, M.T., Sears, C.L. The microbial landscape of colorectal cancer. Nat Rev Microbiology 22, 240–254 (2024).

Holt RA. Oncomicrobial vaccines: The potential for a Fusobacterium nucleatum vaccine to improve colorectal cancer outcomes. Cell Host Microbe. 2023 Jan 11;31(1):141-145. 

Huber, AR,  Pleguezuelos-Manzano, C , Puschhof, J et al Improved detection of colibactin-induced mutations by genotoxic E. coli in organoids and colorectal cancer. Cancer Cell, Volume 42, Issue 3, 2024, Pages 487-496.e6, 


Caley LR, Wood HM, Bottomley D, Fuentes Balaguer A, Wilkinson L, Dyson J, Young C, White H, Benton S, Brearley M, Quirke P, Peckham DG. The gut microbiota in adults with cystic fibrosis compared to colorectal cancer. J Cyst Fibros. 2023 Dec 15:S1569-1993(23)01728-9

Mima K, Hamada T, Inamura K, Baba H, Ugai T, Ogino S. The microbiome and rise of early-onset cancers: knowledge gaps and research opportunities. Gut Microbes. 2023 Dec;15(2):2269623. Epub 2023 Oct 30

Robinson, A, Allen-Vercoe, E Strain specificity in fusobacterial co-aggregation with colorectal cancer-relevant species Anaerobe, August 2023, Volume 82.

Allen-Vercoe E, Daigneault MC, Vancuren SJ, Cochrane K, O’Neal LL, Sankaranarayanan K, Lawson PA. Holtiella tumoricola gen. nov. sp. nov., isolated from a human clinical sample. Int J Syst Evol Microbiol. 2023 Jul;73(7).

El Tekle G, Garrett WS. Bacteria in cancer initiation, promotion and progression. Nat Rev Cancer. 2023 Sep;23(9):600-618.  Epub 2023 Jul 3.

Birch RJ, Peckham D, Wood HM, Quirke P, Konstant-Hambling R, Brownlee K, Cosgriff R, Consortium GER, Burr N, Downing A. The risk of colorectal cancer in individuals with mutations of the cystic fibrosis transmembrane conductance regulator (CFTR) gene: An English population-based study – Journal of Cystic Fibrosis J Cyst Fibros. 2023 May;22(3):499-504.

Porter, LD, Goodman KS, Mailman, J Garrett, WS. Patient Advocates and Researchers as Partners in Cancer Research: A Winning Combination. Am Soc Clin Oncol Educ Book 43, e100035(2023).

Baraibar I, García A, Salvà F, Ros J, et al Impact of the COVID-19 pandemic in the early-onset colorectal cancer  Transl Oncol. 2023 Jun;32:101668. Epub 2023 Apr 5. 

Tsai PC, Lee TH, Kuo KC, Su FY, Lee TM, Marostica E, Ugai T, Zhao M, Lau MC, Väyrynen JP, Giannakis M, Takashima Y, Kahaki SM, Wu K, Song M, Meyerhardt JA, Chan AT, Chiang JH, Nowak J, Ogino S, Yu KH. Histopathology images predict multi-omics aberrations and prognoses in colorectal cancer patients. Nat Commun. 2023 Apr 13;14(1):2102.

Wilde, J, Allen-Vercoe, E. Characterizing prophages in the genus Fusobacterium. Anaerobe, April 2023, Vol 80.

Giannakis, M and Ng, K. A common cancer at an uncommon age Science. 16 March 2023, Vol 379Issue 6637, pp. 10881090.

Ugai T, Akimoto N, Haruki K, Harrison TA, Cao Y, Qu C, Chan AT, Campbell PT, Berndt SI, Buchanan DD, Cross AJ, Diergaarde B, Gallinger SJ, et al Prognostic role of detailed colorectal location and tumor molecular features: analyses of 13,101 colorectal cancer patients including 2994 early-onset cases. J Gastroenterol. 2023 Mar;58(3):229-245. Epub 2023 Jan 17.

Bose, M., Wood, H.M., Young, C. et al. Analysis of an Indian colorectal cancer faecal microbiome collection demonstrates universal colorectal cancer-associated patterns, but closest correlation with other Indian cohorts  BMC Microbiology 23, 52 (2023).

Ugai, Tomotaka MD, PhD, Haruki, Koichiro MD, PhD, Harrison, Tabitha A. MPH, Cao, Yin ScD et. al Molecular Characteristics of Early-Onset Colorectal Cancer According to Detailed Anatomical locations: Comparison with Later-Onset Cases The American Journal of Gastroenterology  January 30, 2023


Wang, FUgai, THaruki, K, et al. Healthy and unhealthy plant‐based diets in relation to the incidence of colorectal cancer overall and by molecular subtypes Clinical and  Translational Medicine202212:e893.

Fang L, Ford-Roshon D, Russo M, O’Brien C, Xiong X, Gurjao C, Grandclaudon M, Raghavan S, Corsello SM, Carr SA, Udeshi ND, Berstler J, Sicinska E, Ng K, Giannakis M. RNF43 G659fs is an oncogenic colorectal cancer mutation and sensitizes tumor cells to PI3K/mTOR inhibition Nature Communications. 2022 Jun 8;13(1):3181. 

Puschhof, J and Sears, C. Microbial metabolites damage DNA: Unexpected members of the gut microbiota produce diverse host cell genotoxins. Science. 27 Oct 2022, Vol 378, Issue 6618, pp. 358-359.

Inamura K, Hamada T, Bullman S, Ugai T, Yachida S, Ogino S. Cancer as microenvironmental, systemic and environmental diseases: opportunity for transdisciplinary microbiomics science. Gut. 2022 Jul 12:gutjnl-2022-327209. doi: 10.1136/gutjnl-2022-327209. Epub ahead of print. PMID: 35820782.

Clay SL, Fonseca-Pereira D, Garrett WS. Colorectal cancer: the facts in the case of the microbiota. Journal of  Clinical Investigation. 2022 Feb 15;132(4):e155101.

Porter, LD The Importance of Patient Engagement to Improve Healthcare Research and Safety. Global Journal on Quality and Safety in Healthcare 1 May 2022; 5 (2): 27–30. doi:

Arima K, Zhong R, Ugai T, Zhao M, Haruki K, Akimoto N, Lau MC, Okadome K, Mehta RS, Väyrynen JP, Kishikawa J, Twombly TS, Shi S, Fujiyoshi K, Kosumi K, Ogata Y, Baba H, Wang F, Wu K, Song M, Zhang X, Fuchs CS, Sears CL, Willett WC, Giovannucci EL, Meyerhardt JA, Garrett WS, Huttenhower C, Chan AT, Nowak JA, Giannakis M, Ogino S. Western-style Diet, pks Island-Carrying Escherichia coli, and Colorectal Cancer: Analyses from Two Large Prospective Cohort Studies. Gastroenterology. 2022 Jun 23:S0016-5085(22)00672-2. doi: 10.1053/j.gastro.2022.06.054. Epub ahead of print. PMID: 35760086.

Drewes JL, Chen J, Markham NO, Knippel RJ, Domingue JC, Tam AJ, Chan JL, Kim L, McMann M, Stevens C, Dejea CM, Tomkovich S, Michel J, White JR, Mohammad F, Campodonico VL, Heiser CN, Wu X, Wu S, Ding H, Simner P, Carroll K, Shrubsole MJ, Anders RA, Walk ST, Jobin C, Wan F, Coffey RJ, Housseau F, Lau KS, Sears CL. Human colon cancer-derived Clostridioides difficile strains drive colonic tumorigenesis in mice. Cancer Discov. 2022 Jun 9:candisc.1273.2021-10-4 15:58:09.930. doi: 10.1158/2159-8290.CD-21-1273. Epub ahead of print. PMID: 35678528.

Fang L, Ford-Roshon D, Russo M, O’Brien C, Xiong X, Gurjao C, Grandclaudon M, Raghavan S, Corsello SM, Carr SA, Udeshi ND, Berstler J, Sicinska E, Ng K, Giannakis M. RNF43 G659fs is an oncogenic colorectal cancer mutation and sensitizes tumor cells to PI3K/mTOR inhibition. Nat Commun. 2022 Jun 8;13(1):3181. doi: 10.1038/s41467-022-30794-7. PMID: 35676246; PMCID: PMC9177965.

Li R, Ugai T, Xu L, Zucker D, Ogino S, Wang M. Utility of Continuous Disease Subtyping Systems for Improved Evaluation of Etiologic Heterogeneity. Cancers (Basel). 2022 Apr 2;14(7):1811. doi: 10.3390/cancers14071811. PMID: 35406583; PMCID: PMC8997600.


Despins CA, Brown SD, Robinson AV, Mungall AJ, Allen-Vercoe E, Holt RA. Modulation of the Host Cell Transcriptome and Epigenome by Fusobacterium nucleatum. mBio. 2021 Oct 26;12(5):e020622.

Brennan, CA, Clay, SL, Lavoie, SL, Bae, S, Lang, JK, Fonseca-Pereira, D, Rosinski, KG, Ou, N, Glickman, JN, Garrett, WS.  Fusobacterium nucleatum drives a pro-inflammatory intestinal microenvironment through metabolite receptor-dependent modulation of IL-17 expressionGut Microbes 13:1, 2021

Wang Y, Nguyen LH, Mehta RS, Song M, Huttenhower C, Chan AT. Association Between the Sulfur Microbial Diet and Risk of Colorectal Cancer. JAMA Network Open. 2021;4(11).

Borozan, I, Zaidi, SH, Harrison, TA, Phipps, AI, Zheng, J, Lee, S, TrinhQM, Steinfelder, RS, AdamsJ, Banbury, BL, BerndtSI, Brezina, S, Buchanan, DD, Bullman, S, Cao, Y, Farris, AB, Figueiredo, JC,  Giannakis, M, Heisler, LE, HopperJL, Lin, Y, LuoX, NishiharaR, MardisER, PapadopoulosN, Qu, C, Reid, EG, Thibodeau, SN, Harlid, S, Um, CY, Hsu, L, Gsur, A, Campbell, PT, Gallinger, S, Newcomb, PA, Ogino, S, Sun, W, Hudson, TJ, Ferretti, V and Peters, U. Molecular and pathology features of colorectal tumors and patient outcomes are associated with Fusobacterium nucleatum and its subspecies animalis. Cancer Epidemiology Biomarkers & Prevention November 4, 2021. 

Ugai, T, Väyrynen, JP, Lau, MC, Borowsky, J, Akimoto, N, Väyrynen, SA, Zhao, M, Zhong, R, Haruki, K, Dias Costa, A, Fujiyoshi, K, Arima, K, Wu, K, Chan, AT, Cao, Y,  Song, M, Fuchs,CS, Wang, M, Lennerz, JK, Ng, K,  Meyerhardt, JA, Giannakis, M, Nowak, JA, Ogino, S. Immune cell profiles in the tumor microenvironment of early-onset, intermediate-onset, and later-onset colorectal cancer. Cancer Immunology, Immunotherapy September 16, 2021.

Gurjao C, Zhong R, Haruki K, Li YY, Spurr L F, Lee-Six H, Reardon B, Ugai T, Zhang X, Cherniack AD, Song M, Van Allen EM, Meyerhardt JA, Nowak JA, Giovannucci EL, Fuchs CS, Wu K, Ogino S,  Giannakis M. Discovery and Features of an Alkylating Signature in Colorectal Cancer. Cancer Discovery August 16 2021

Puschhof J, Pleguezuelos-Manzano C, Martinez-Silgado A, Akkerman N, Saftien A, Boot C, de Waal A, Beumer J, Dutta D, Heo I, Clevers H. Intestinal organoid cocultures with microbes. Nature Protocols. 2021 Aug 11. Epub ahead of print. PMID: 34381208.

Sikavi DR, Nguyen LH, Haruki K, Ugai T, Ma W, Wang DD, Thompson KN, Yan Y, Branck T, Wilkinson JE, Akimoto N, Zhong R, Lau MC, Mima K, Kosumi K, Morikawa T, Rimm EB, Garrett WS, Izard J, Cao Y, Song M, Huttenhower C, Ogino S, Chan AT. The Sulfur Microbial Diet and Risk of Colorectal Cancer by Molecular Subtypes and Intratumoral Microbial Species in Adult Men.  Clinical and  Translational Gastroenterology. 2021 Aug 1;12(8)

DeStefano Shields CE, White JR, Chung L, Wenzel A, Hicks JL, Tam AJ, Chan JL, Dejea CM, Fan H, Michel J, Maiuri AR, Sriramkumar S, Podicheti R, Rusch DB, Wang H, De Marzo AM, Besharati S, Anders RA, Baylin SB, O’Hagan HM, Housseau F, Sears CL. Bacterial-driven inflammation and mutant BRAF expression combine to promote murine colon tumorigenesis that is sensitive to immune checkpoint therapy. Cancer Discovery. 2021 July.

Renwick S, Ganobis CM, Elder RA, Gianetto-Hill C, Higgins G, Robinson AV, Vancuren SJ, Wilde J, Allen-Vercoe E. Culturing Human Gut Microbiomes in the Laboratory. Annual Review Microbiology. 2021 May 26. doi: 10.1146/annurev-micro-031021-084116.

Beghini F, McIver LJ, Blanco-Míguez A, Dubois L, Asnicar F, Maharjan S, Mailyan A, Manghi P, Scholz M, Thomas AM, Valles-Colomer M, Weingart G, Zhang Y, Zolfo M, Huttenhower C, Franzosa EA, Segata N. Integrating taxonomic, functional, and strain-level profiling of diverse microbial communities with bioBakery 3. Elife. 2021 May 4;10:e65088.

Brennan CA, Nakatsu G, Gallini Comeau CA, Drew DA, Glickman JN, Schoen RE, Chan AT, Garrett WS. Aspirin Modulation of the Colorectal Cancer-Associated Microbe Fusobacterium nucleatum. mBio. 2021 Apr 6;12(2):e00547-21.

Rosendahl Huber A, Pleguezuelos-Manzano C, Puschhof J. A bacterial mutational footprint in colorectal cancer genomes. British Journal of Cancer. 2021 May;124(11):1751-1753. doi: 10.1038/s41416-021-01273-5. Epub 2021 Mar 19.

Young C, Wood HM, Fuentes Balaguer A, Bottomley D, Gallop N, Wilkinson L, Benton SC, Brealey M, John C, Burtonwood C, Thompson KN, Yan Y, Barrett JH, Morris EJA, Huttenhower C, Quirke P. Microbiome Analysis of More Than 2,000 NHS Bowel Cancer Screening Programme Samples Shows the Potential to Improve Screening Accuracy. Clinical Cancer Research. 2021 Mar 3.

Young C, Wood HM, Seshadri RA, Van Nang P, Vaccaro C, Melendez LC, Bose M, Van Doi M, Piñero TA, Valladares CT, Arguero J, Balaguer AF, Thompson KN, Yan Y, Huttenhower C, Quirke P. The colorectal cancer-associated faecal microbiome of developing countries resembles that of developed countries. Genome Medicine. 2021 Feb 16;13(1):27.

Nevo D, Ogino S, Wang M. Reflection on modern methods: causal inference considerations for heterogeneous disease etiology. International Journal of Epidemiol. 2021 Jan 23.

Väyrynen JP, Haruki K, Lau MC, Väyrynen SA, Zhong R, Dias Costa A, Borowsky J, Zhao M, Fujiyoshi K, Arima K, Twombly TS, Kishikawa J, Gu S, Aminmozaffari S, Shi S, Baba Y, Akimoto N, Ugai T, Da Silva A, Guerriero JL, Song M, Wu K, Chan AT, Nishihara R, Fuchs CS, Meyerhardt JA, Giannakis M, Ogino S, Nowak JA. The Prognostic Role of Macrophage Polarization in the Colorectal Cancer Microenvironment. Cancer Immunol Res. 2021 Jan;9(1):8-19.


Babic A, Zhang X, Morales-Oyarvide V, Yuan C, Khalaf N, Khalili H, Lochhead P, Chan AT, Ogino S, Wolpin BM, Wu K, Fuchs CS, Giovannucci EL, Stampfer MJ, Ng K. Acid-suppressive medications and risk of colorectal cancer: results from three large prospective cohort studies. Br J Cancer. 2020 Sep;123(5):844-851.

Pleguezuelos-Manzano C, Puschhof J, van den Brink S, Geurts V, Beumer J, Clevers H. Establishment and Culture of Human Intestinal Organoids Derived from Adult Stem Cells. Curr Protoc Immunol. 2020 Sep;130(1):e106. 

Väyrynen JP, Lau MC, Haruki K, Väyrynen SA, Dias Costa A, Borowsky J, Zhao M, Fujiyoshi K, Arima K, Twombly TS, Kishikawa J, Gu S, Aminmozaffari S, Shi S, Baba Y, Akimoto N, Ugai T, Da Silva A, Song M, Wu K, Chan AT, Nishihara R, Fuchs CS, Meyerhardt JA, Giannakis M, Ogino S, Nowak JA. Prognostic Significance of Immune Cell Populations Identified by Machine Learning in Colorectal Cancer Using Routine Hematoxylin and Eosin-Stained Sections. Clin Cancer Res. 2020 Aug 15;26(16):4326-4338. 

Yan Y, Nguyen LH, Franzosa EA, Huttenhower C. Strain-level epidemiology of microbial communities and the human microbiome. Genome Med. 2020 Aug 13;12(1):71. doi: 10.1186/s13073-020-00765-y. PMID: 32791981; PMCID: PMC7427293. 

Serna G, Ruiz-Pace F, Hernando J, Alonso L, Fasani R, Landolfi S, Comas R, Jimenez J, Elez E, Bullman S, Tabernero J, Capdevila J, Dienstmann R, Nuciforo P. Fusobacterium nucleatum persistence and risk of recurrence after preoperative treatment in locally advanced rectal cancer. Ann Oncol. 2020 Jun 20:S0923-7534(20)39890-2. 

Beumer J, Puschhof J, Bauzá-Martinez J, Martínez-Silgado A, Elmentaite R, James KR, Ross A, Hendriks D, Artegiani B, Busslinger GA, Ponsioen B, Andersson-Rolf A, Saftien A, Boot C, Kretzschmar K, Geurts MH, Bar-Ephraim YE, Pleguezuelos-Manzano C, Post Y, Begthel H, van der Linden F, Lopez-Iglesias C, van de Wetering WJ, van der Linden R, Peters PJ, Heck AJR, Goedhart J, Snippert H, Zilbauer M, Teichmann SA, Wu W, Clevers H. High-Resolution mRNA and Secretome Atlas of Human Enteroendocrine Cells. Cell. 2020 Jun 11;181(6):1291-1306.e19 

McCuaig S, Barras D, Mann EH, Friedrich M, Bullers SJ, Janney A, Garner LC, Domingo E, Koelzer VH, Delorenzi M, Tejpar S, Maughan TS, West NR, Powrie F. The Interleukin 22 Pathway Interacts with Mutant KRAS to Promote Poor Prognosis in Colon CancerClinical Cancer Research. 19 May 2020;26:4313–25. 

Fujiyoshi K, Chen Y, Haruki K, Ugai T, Kishikawa J, Hamada T, Liu L, Arima K, Borowsky J, Väyrynen JP, Zhao M, Lau MC, Gu S, Shi S, Akimoto N, Twombly TS, Drew DA, Song M, Chan AT, Giovannucci EL, Meyerhardt JA, Fuchs CS, Nishihara R, Lennerz JK, Giannakis M, Nowak JA, Zhang X, Wu K, Ogino S. Smoking Status at Diagnosis and Colorectal Cancer Prognosis According to Tumor Lymphocytic Reaction. JNCI Cancer Spectr. 2020 May 14;4(5):pkaa040. doi: 10.1093/jncics/pkaa040. PMID: 32923934; PMCID: PMC7477375. 

Haruki K, Kosumi K, Hamada T, Twombly TS, Väyrynen JP, Kim SA, Masugi Y, Qian ZR, Mima K, Baba Y, da Silva A, Borowsky J, Arima K, Fujiyoshi K, Lau MC, Li P, Guo C, Chen Y, Song M, Nowak JA, Nishihara R, Yanaga K, Zhang X, Wu K, Bullman S, Garrett WS, Huttenhower C, Meyerhardt JA, Giannakis M, Chan AT, Fuchs CS, Ogino S. Association of autophagy status with amount of Fusobacterium nucleatum in colorectal cancerJ Pathol. 2020 Apr;250(4):397-408. 

Haruki K, Kosumi K, Li P, Arima K, Väyrynen JP, Lau MC, Twombly TS, Hamada T, Glickman JN, Fujiyoshi K, Chen Y, Du C, Guo C, Väyrynen SA, Dias Costa A, Song M, Chan AT, Meyerhardt JA, Nishihara R, Fuchs CS, Liu L, Zhang X, Wu K, Giannakis M, Nowak JA, Ogino S. An integrated analysis of lymphocytic reaction, tumour molecular characteristics, and patient survival in colorectal cancer. Br J Cancer. 2020 Apr;122(9):1367-1377. 

Nguyen LH, Ma W, Wang DD, Cao Y, Mallick H, Gerbaba TK, Lloyd-Price J, Abu-Ali G, Hall AB, Sikavi D, Drew DA, Mehta RS, Arze C, Joshi AD, Yan Y, Branck T, DuLong C, Ivey KL, Ogino S, Rimm EB, Song M, Garrett WS, Izard J, Huttenhower C, Chan AT. Association Between Sulfur-Metabolizing Bacterial Communities in Stool and Risk of Distal Colorectal Cancer in Men. Gastroenterology. 2020 Apr;158(5):1313-1325. 

Lavoie S, Chun E, Bae S, Brennan CA, Comeau CAG, Lang JK, Michaud M, Hoveyda HR, Fraser GL, Fuller MH, Layden BT, Glickman JN, Garrett WS. Expression of FFAR2 by Dendritic Cells Prevents Their Expression of IL27 and is Required For Maintenance of Mucosal Barrier and Immune Response Against Colorectal Tumors in Mice. Gastroenterology. 2020. Apr;158(5):1359-1372.e9. 

Yan Y, Drew DA, Markowitz A, Lloyd-Price J, Abu-Ali G, Nguyen LH, Tran C, Chung DC, Gilpin KK, Meixell D, Parziale M, Schuck M, Patel Z, Richter JM, Kelsey PB, Garrett WS, Chan AT, Stadler ZK, Huttenhower C. Structure of the mucosal and stool microbiome in Lynch syndrome. Cell Host & Microbe. 2020. Apr 8;27(4):585-600.e4 

Pleguezuelos-Manzano C*, Puschhof J*, Rosendahl Huber A*, van Hoeck A​, Wood, H, Nomburg JL., Gurjao C, Manders F, Dalmasso G,  Stege P​, Paganelli F, Geurts MH, Beumer J, Mizutani T, van der Linden R​, van Elst S​, The Genomics England Consortium​, Top J, Willems R​, Giannakis M​, Bonnet R​, Quirke P​, Meyerson M​, Cuppen E, van Boxtel R​, Clevers H. Mutational signature in colorectal cancer induced by genotoxic pks+ E. coli. Nature, 2020 Apr;580(7802):269-273


Gurjao C, Liu D, Hofree M, AlDubayan SH, Wakiro I, Su MJ, Felt K, Gjini E, Brais LK, Rotem A, Rosenthal MH, Rozenblatt-Rosen O, Rodig S, Ng K, Van Allen EM, Corsello SM, Ogino S, Regev A, Nowak JA, Giannakis M. Intrinsic Resistance to Immune Checkpoint Blockade in a Mismatch Repair-Deficient Colorectal Cancer. Cancer Immunol Res. 2019;7:1230-1236.